Versatility of Acyl-Acyl Carrier Protein Synthetases
نویسندگان
چکیده
منابع مشابه
Versatility of acyl-acyl carrier protein synthetases.
The acyl carrier protein (ACP) requires posttranslational modification with a 4'-phosphopantetheine arm for activity, and this thiol-terminated modification carries cargo between enzymes in ACP-dependent metabolic pathways. We show that acyl-ACP synthetases (AasSs) from different organisms are able to load even, odd, and unnatural fatty acids onto E. coli ACP in vitro. Vibrio harveyi AasS not o...
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The enzymes catalysing the initial stage of the 8-oxidation of fatty acids, the acyl-CoA synthetases, have been classified into four groups based on specificity. These are: the short-chain (acetyl-CoA synthetase; EC 6.2.1 .l), medium-chain (butyryl-CoA synthetase; EC 6.2.1.2) and the long-chain fatty acyl-CoA synthetase (acyl-CoA synthetase; EC 6.2.1.3), which are ATP-dependent and follow the r...
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/3-Hydroxyacyl acyl carrier protein (ACP) dehydrase has been purified 2900-fold from extracts of Escherichia coli. The enzyme catalyzes the reversible dehydration of /3-hydroxyacyl-ACP thioesters to yield specifically tram-2-enoylACP products. It is active with frans-2-enoyl-ACP thioesters of chain lengths from 4 through 16 carbon atoms. The enzyme catalyzes the hydration of cis-5frans-2-dodeca...
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Stearyl acyl carrier protein, chemically prepared from 1-W stearic acid and Escherichia co2i acyl carrier protein, is desaturated to oleic acid by extracts of photoauxotrophic Euglena gracilis and of spinach. The soluble stearyl acyl carrier protein desaturase system from Eug~ena has been separated into three components, a reduced triphosphopyridine nucleotide oxidase, the desaturase, and a non...
متن کاملP-Ketoacyl-Acyl Carrier Protein Synthetase
/3-Ketoacyl-acyl carrier protein (ACP) synthetase catalyzes the condensation reaction of fatty acid synthesis in Escherichia coli. The homogeneous enzyme reacts with hexanoylCoA to form hexanoyl-enzyme which was isolated and characterized. Hexanoyl-enzyme contains 2 mol of hexanoate/mol of enzyme (molecular weight 66,000); it is labile at alkaline pH, and it reacts with neutral hydroxylamine to...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2014
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2014.08.015